At 5X magnification, the depth of field is razor thin - just a millimeter or so. On top of that, the working distance is a mere 41mm (just 4.1cm away from the insect!) These shots were even more painfully hard to take.
Canon 5D Mark II, Canon Speedlite 580EXII, Canon MP-E 65mm, 65mm, 1/640 seconds @ f/8.0, ISO 3200
Canon EOS 5D Mark II
1/640 second
F/8.0
ISO 1600
65 mm